Biotinylate
verb
verb ·Rare ·Advanced level
Definitions
Verb
- 1 To attach a biotin residue to a biological macromolecule in order to label it
"After 30 min, the reaction was supplemented to a final volume of 25 μL with 5 units of Klenow fragment (3′→5′ exo-) DNA polymerase (NEB, USA) and 5 μM of biotin-14-dUTP, dATP, dGTP and dCTP and incubated at 37 °C for another 30 min to fill in the overhangs and biotinylate the fragmented DNA."
Example
More examples"After 30 min, the reaction was supplemented to a final volume of 25 μL with 5 units of Klenow fragment (3′→5′ exo-) DNA polymerase (NEB, USA) and 5 μM of biotin-14-dUTP, dATP, dGTP and dCTP and incubated at 37 °C for another 30 min to fill in the overhangs and biotinylate the fragmented DNA."
Etymology
From biotinyl + -ate.
More for "biotinylate"
Data sourced from Wiktionary, WordNet, CMU, and other open linguistic databases. Updated March 2026.