Anticytokine

Briefly, the anticytokine antibody-conjugated beads were added to individual wells of a flat bottom plate (96-well), 50 μl of prediluted standards or samples was added after washing, and the plate was incubated at room temperature with shaking at 850 rpm for 1 h.

We therefore hypothesized that, analogous to the mouse model, humans with Lyme arthritis express a predominant Th1 pattern in their affected joints. We also addressed the issue of whether the cytokine pattern found in the joints can be manipulated by cytokines or anticytokines.