Digestate

The sample was digested with sulphuric acid, neutralized, distilled, and Nesslerized. At the same time, tests were run [...] to obtain concordant results by direct Nesslerization of the ammonia formed by the distillation of the digestate.

Digestates were quantitatively transferred and diluted to a final volume of 50 ml with 1% HOT. Diluted digestates of bluegill samples were stored in preleached (Karin et al. 1975) conventional polyethylene bottles (Moody and Lindstrom 1977) prior to analysis.