Gladiolin

Gladiolin is structurally related to etnangien, a highly unstable antibiotic from Sorangium cellulosum that is also active against Mycobacteria.

The configuration of all but one of the stereogenic centers of gladiolin has previously been elucidated using a combination of NOESY NMR experiments and predictive sequence analysis of the polyketide synthase responsible for its assembly.

In this study, a previously uncharacterised trans-AT PKS subunit interface between a ketoreductase and a methyltransferase domain (KR/MT junction) is examined from the gladiolin trans-AT PKS.