Immunopure

However, when the anti- RBP IgG was affinity-purified on immobilized pure RBP, the resultant antibody was immunopure and no longer recognized albumin (Fig.

The resulting immunopure MHC II peptide monomers are enzymatically biotinylated and multimerized with PE strep tavidin.

By means of an N-terminal His₆-peptide tag and IDA chromatography immunopure DR52b/NY-ESO-1_(119–143) monomers were produced and with these PE labeled multimers, which exhibited dramatically improved staining a cloned ESO-specific CD4+ T cells compared to conventional DR52b/NY-ESO-1₁₁₉₋₁₄₃ multimers (Figures 5A,C-E).