Intracerebroventricularly

Pups were anesthetized with 2.5% halothane and intracerebroventricularly infused with vehicle (dimethyl sulfoxide [DMSO], Sigma-Aldrich, USA) or 150 nmol of AS601245 (Alexis Biochemicals, Lausen, Switzerland) dissolved in DMSO into the right cerebral hemisphere 30 minutes prior to HI using a 30-gauge needle with a 5-μL Hamilton syringe (infusion rate 1 μL/min) injections.