Irresponsiveness

As per the guidelines, all animals with severe CS were treated with clinically appropriate anticonvulsants, and mandatory euthanization (with pentobarbital anesthesia) was performed if animals exhibited severe, refractory CS and/or presented in poor physical condition, defined as persistent immobility, lack of eating or drinking, irresponsiveness to touch, loss of the righting reflex, and/or a substantial reduction in body weight (≥20% of baseline level).