Pancoronavirus

In 2005, an optimized pancoronavirus reverse transcription–PCR assay was used to explore the incidence of HCoV-NL63 infection in children in Belgium who had a diagnosis of respiratory tract infection.

We designed a novel pancoronavirus RT-PCR in which we modified the coronavirus consensus RT-PCR primers based on an alignment of the HCoV-NL63 sequence and the corresponding sequences of 13 other coronaviruses.

Pancoronavirus RNA was observed in seven cases among 98 nasal swab samples.