Polyclonals

Blots were probed with anti-phospho-ERK1/2 followed by anti-ERK1/2 antibodies (Cell Signaling, 9101 and 9102, rabbit polyclonals) or anti-CaSR antibody (rabbit polyclonal against LRG epitope (identified as antigenic in [24 ]), independently custom-generated by Genemed Synthesis, Inc. for us in 2002, first cited in [25 ]), and species-appropriate secondary antibodies (ECL, HRP-conjugated, GE Healthcare).