Preculture

Second, some of the precultures used by Iglesias-Rodriguez et al., particularly those in high-CO₂ treatments, may have experienced nutrient limitation at the time of transfer to the experimental flasks.

For fresh, nonfrozen hepatocytes precultured for 24 h prior to being plated on collagen, the albumin secretion rate was 0.88 ± 0.62 mg/ml/h.