Qpcr

Similar experiments in humans have been hampered because so far very few anti-human V_β (hV_β) monoclonal antibodies are available, although some important data have been generated with a labor-intensive quantitative polymerase chain reaction (QPCR).

Quantitative or Real-Time PCR Real-time PCR, also called qPCR, is known as the second generation of PCR because it could be used to amplify and simultaneously quantify several target DNA molecules.

This cheap and simple chemistry is optimal for single plex qPCRs. For multiplex qPCRs, specific chemistries which make use of probes are required.