Transfucosylation

Additionally, for Mfuc5, a reaction using a suspension of 33.7 g/L citrus peel xyloglucan (5.55 mM bound fucose), 140 mM lactose and 56 mM phosphate-citrate buffer (pH 7) was done at 30°C. The final enzyme concentrations were as for the transfucosylation experiment described above.