Unexcised

The presence of the integrated T-DNA into the rice genome was reassessed by PCR with the gene specific primer sets P35SF/HTPR and P35SF/TCR. PCR results indicated that the 1,256-bp P35S-hpt fragment could be detected in all of five plant lines, while the 1,480-bp P35S-NtTC fragment was detectable in only two plant lines, indicating that these transgenic lines contained multiple T-DNAs with excised and unexcised sequences.