Zymographies

After electrophoresis, gelatin zymographies were incubated for 24 hours at 37°C in two developing buffers: Activator buffer containing 2 mmol/L CaCl 2 ;, Tris-HCl buffer (50 mmol/L; pH 7.4), containing 1.5% Triton X-100 and 0.02% Na Azide plus inhibitor buffer Tris-HCl buffer (50 mmol/L; pH 7.4), containing 1.5% Triton X-100 and 0.02% Na Azide plus 2 mmol/L EDTA to inhibit any gelatinase activity.