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2 total sentences available.
To develop an animal model for antimyeloperoxidase (MPO)-associated necrotizing crescentic glomerulonephritis (NCGN), we immunized Brown Norway rats with MPO and localized a neutrophil lysosomal enzyme extract, primarily consisting of MPO and elastinolytic enzymes, plus H₂O₂, the substrate of MPO, to the glomerular basement membrane (GBM).
Source: wiktionary
They were then incubated with the first antibodies: rabbit antimyeloperoxidase antibodies (1:200, RB-373; Thermo Fisher Scientific, Waltham, Massachusetts) for neutrophil staining, rat antimouse Mac-3 antibodies (1:500; BioLegend, San Diego, California) for macrophage staining, rabbit anti-CD3 polyclonal antibodies (1:100; ab5690, Abcam, Cambridgeshire, UK) and rat anti-CD19 monoclonal antibodies (1:500; ab25232, Abcam, Cambridgeshire, UK) for lymphocyte staining.
Source: wiktionary
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