After blocking with 5% nonfat milk in TBST for 1 hour, the membranes were incubated with the primary antibody to PAK4 (Abcam, Cambridge, MA), human equilibrative nucleoside transporter 1 (hENT1; Proteintech, Chicago, IL), excision repair cross-complementing rodent repair deficiency, complementation group 1 (ERCC1, Santa Cruz Biotech, Santa Cruz, CA), PAK1, phosphorylated epidermal growth factor receptor, EGFR, pAkt, Akt, pErk, Erk, and β-actin (Cell Signaling).
Source: wiktionary