RT-PCR primers for the identification of active HERV loci were designed from the sequences of predicted/expected loci and incorporated 0 to 3 ambiguous nucleotides taking the other homologous loci into account. […] Locus-specific primers were set in interexons. Those sequences and PCR conditions were as follows: […]
Source: wiktionary
The results of this analysis showed that there are significant differences between all the groups of pathologies studied regarding the level of expression of the various interexons, understanding significance in each group as the statistical difference found at least between a pair of pathologies concerning the values of expression in every interexon.
Source: wiktionary
Given the technical inability to obtain real quantitative data for the expression levels of all Ikaros isoforms, a different protocol was used to characterize the expression profile of each interexon based on qRT-PCR.
Source: wiktionary