Briefly, dsNlRan (stock solution estimated at 4.000 mg/mL), dsGFP (estimated at 4.000 mg/mL, negative control), or double distilled water (blank control) was injected into the thorax between the mesocoxa and the hind coxa of third-instar nymphs (2-day old), fourth-instar nymphs (2-day old) or fifth-instar nymphs (3-day old) in volumes of 0.025 μL, 0.05 μL and 0.1 μL, respectively, with different doses.
Source: wiktionary