It is also useful when using degenerate primers which may misprime and cause nonspecific products.
Source: wiktionary
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It is also useful when using degenerate primers which may misprime and cause nonspecific products.
Source: wiktionary
As described previously, primers are apt to dimerize or misprime, particularly in the absence of specific target.
Source: wiktionary
Aberrant annealing has also been observed — either due to the use of the incorrect tRNA to misprime reverse transcription (Colicelli and Goff 1986a, b; Wakefield et al. 1995) or due to the priming of cellular mRNAs with short “tRNA-like” primers (Taylor and Cywinski 1984).
Source: wiktionary
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