Mononuclear cells (5×10 6 ) were labeled with preconjugated mouse anti-human monoclonal antibodies: CD34 conjugated to allophycocyanin, CD309 (VEGF receptor-2 [VEGFR2]/kinase insert domain-containing receptor [KDR]) to fluorescein isothiocyanate (BD Biosciences, USA), and CD45 to peridinin chlorophyll protein-cyanine for 20 min at room temperature.
Source: wiktionary