Before inoculation, the growth media were prereduced in an anaerobic jar (Anaero-Gen™, Oxoid Inc.,UK), supplemented with stock solutions of freshly prepared and filter-sterilized Cys-HCl (concentration in the medium 0.5 g/L) and autoclaved sodium thioglycolate (concentration in the medium 0.5 g/L) as reducing agents.
Source: wiktionary