Similar experiments in humans have been hampered because so far very few anti-human V_β (hV_β) monoclonal antibodies are available, although some important data have been generated with a labor-intensive quantitative polymerase chain reaction (QPCR).
Source: wiktionary
Quantitative or Real-Time PCR Real-time PCR, also called qPCR, is known as the second generation of PCR because it could be used to amplify and simultaneously quantify several target DNA molecules.
Source: wiktionary
This cheap and simple chemistry is optimal for single plex qPCRs. For multiplex qPCRs, specific chemistries which make use of probes are required.
Source: wiktionary