Ultrafiltrated

The retentate was diluted in lipid assay buffer (10 mM KH 2 PO 4, 100 mM NaCl, 2,7 mM KCl) [36 ] and ultrafiltrated again until the Imidazol concentration was less than 1 mM. Lipid pull-down assay Vesicles were generated essentially as described before.