Mononuclear cells isolated using lymphocyte separation medium (EuroBio, Courtaboeuf, France) were seeded in complete α-MEM medium supplemented under osteoclastogenic conditions with 30 ng mL -1 mRANKL and 25 ng mL -1 recombinant mouse macrophage-colony stimulating factor (PAP; SFR Biosciences), in a so-called differentiation medium.
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