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Next Reports Eight Calves Cloned from Somatic Cells of a Single Adult Yoko Kato, Tetsuya Tani, Yusuke Sotomaru, Kazuo Kurokawa, Jun-ya Kato, Hiroshi Doguchi, Hiroshi Yasue, Yukio Tsunoda * Eight calves were derived from differentiated cells of a single adult cow, five from cumulus cells and three from oviductal cells out of 10 embryos transferred to surrogate cows (80 percent success).
Source: wiktionary
Eggs were collected 13 to 15 hours after the hCG injection from the oviductal ampulla region under paraffin and were placed in a 400 µl drop of TYH medium (24) equilibrated with 5% CO 2 in air at 37°C. Zona-free eggs were prepared by acidic tyrode according to the method described [B. Hogan, F. Costantini, E. Lacy, Manipulating the Mouse Embryo (Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, 1986)].
Source: wiktionary
[View Larger Version of this Image (106K GIF file)] A fourth surrogate in series C maintained pregnancy after the oviductal transfer of 36 cloned embryos that were derived from fibroblast cultures at passage two.
Source: wiktionary